21 Febbraio 2018

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Are cultured stromal cells a suitable model to study Osteopathic Manipulative Treatment (OMT) "in vitro"? A first experimental approach

Poster presentato a Firenze nel settembre 2012 al congresso Europeo di medicina integrata (ECIM2012)
D. Saverino1, D. Lanaro2, M. Lozia 2, P. Luzzi1, A. Strangio1, G. Donniaquio2, I. Demori31Dipartimento di Medicina Sperimentale (DIMES), University of Genoa, Italy2Istituto Europeo per la Medicina Osteopatica (IEMO), School of Genoa, Italy3Dipartimento di Scienze della Terra, dell´Ambiente e della Vita (DISTAV), University of Genoa,
Osteopathic Manipulative Treatment (OMT) is believed to affect cell shape, proliferation and function, but scientific evidence on the molecular mechanisms underlying these effects are almost at all lacking.Aiming to address this issue with a direct in vitro approach, we tested two different types of cell cultures: stromal vascular cells isolated from rat epididymal fat pads and mouse bone marrow stromal cells. The cells were grown on collagenated glass coverslips and 24 hours after seeding underwent an OMT operatively called biodynamic transformation. The maneuver was performed by Doctors of Osteopathic Medicine of the IEMO in Genoa. Control cells were either left untouched or sham-treated by an individual with no knowledge of OMT. 36 hours after treatment cell viability was checked by Trypan Blue Exclusion Test. Then cells were fixed and stained with a fluorescent monoclonal antibody to tubulin and analyzed by fluorescence microscopy.A specific higher fluorescence was detected in treated cells with respect to controls, showing an apparent cytoskeleton reorganization and indicating that OMT could directly affect the shape of cultured cells. Our preliminary data indicate the possibility to directly affect stromal cell cultures with OMT maneuvers, thus supporting the use of "in vitro" models to study the molecular mechanisms underlying the principles of osteopathic medicine.

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